A HPLC?DAD method for identifying and estimating the content of fucoxanthin, ??carotene and chlorophyll a in brown algal extracts

• Effective extraction of fucoxanthin, ?-carotene and chlorophyll-a from brown algae. Development a simple HPLC method for detecting algae pigments. Rapid identification quantification pigments by HPLC-DAD. Assessment the best techniques to obtain highest yield. Efficient pigment with UAE nine species. Seaweeds are photosynthetic organisms that have high contents The coloration each alga is defined content combination synthesized, which varies among species environmental conditions. most abundant in chlorophylls carotenoids, lipophilic molecules can be used as natural colorants acceptance consumers. In this work, short hands-on time HPLC-DAD identifying estimating algal extracts, specifically chlorophyll was carried out. Using optimized method, screening performed on ethanolic extracts obtained ultrasound-assisted Atlantic coastline: Ascophyllum nodosum, Bifurcaria bifurcata, Fucus spiralis, Himanthalia elongata, Laminaria saccharina, ochroleuca, Pelvetia canaliculata, Sargassum muticum Undaria pinnatifida . results permitted highlight L. saccharina U. promising sources these three target containing total amount 10.5 – 11.5 mg per gram dry weight. Among them, one an added-value compound potential commercially exploited different industries, such food, cosmetic, pharmaceutical sectors. A minimal quantifying extracted macroalgae, developed. Moreover, applied establish relevant terms production.